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Saturday, August 8, 2020 | History

3 edition of Morphological differentiation of colon carcinoma cell lines in rotating wall vessels found in the catalog.

Morphological differentiation of colon carcinoma cell lines in rotating wall vessels

Morphological differentiation of colon carcinoma cell lines in rotating wall vessels

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  • 25 Currently reading

Published by National Aeronautics and Space Administration, National Technical Information Service, distributor in [Washington, DC, Springfield, Va .
Written in English

    Subjects:
  • Cancer.,
  • Cell division.,
  • Culture techniques.,
  • Cytology.,
  • Differentiation (Biology),
  • Epithelium.,
  • Histochemical analysis.,
  • Intestines.,
  • Microbiology.,
  • Microgravity.,
  • Morphology.

  • Edition Notes

    Statementprincipal investigator, J. Milburn Jessup.
    SeriesNASA contractor report -- NASA CR-196780.
    ContributionsUnited States. National Aeronautics and Space Administration.
    The Physical Object
    FormatMicroform
    Pagination1 v.
    ID Numbers
    Open LibraryOL15403872M

    Goodwin TJ, Jessup JM, Wolf DA (): Morphological differentiation of colon carcinoma cell lines HT‐29 & HT‐29KM in rotating‐wall vessels In Vitro. Cell Dev Biol 28A: 47 – Grande D, Pitman MI, Peterson L, Menche D, Klein M (): The repair of experimentally produced defects in rabbit articular cartilage by autologous chondrocyte transplantation.   The tumor encircles the colon and infiltratesinto the wall. Staging is based upon the degree of invasion into Adenocarcinoma of the cecum demonstrates an exophytic growthpattern. The barium enema instills the radiopaque barium sulfate into the colon,producing a contrast with the wall of the colon that highlights any massespresent.

    The first breakthrough in building a 3-D model was reported in the early of s when scientists started to investigate different types of the scaffold (e.g., laminin, collagen type I, collagen IV, and fibronectin) and cocktails of growth factors to improve cell-to-cell and ECM interactions of "static" 3-D models Since then, the main problem with these models has been limitations in the. Functional Tissue Engineering of Articular Cartilage Through Dynamic Loading of Chondrocyte-Seeded Agarose Gels Morphologic Differentiation of Colon Carcinoma Cell Lines HT, and HTKM in Rotating-Wall Vessels,”.

    (b) Rotating-wall vessels provide a dynamic culture environment to the constructs, with low shear stresses and high mass-transfer rates. The vessel walls are rotated at a rate that enables the drag force (F d), centrifugal force (F c) and net gravitational force (F g) on the construct to be balanced; the construct thus remains in a state of. Goodwin TJ, Jessup JM, Wolf DA. Morphologic differentiation of colon carcinoma cell lines HT and HTKM in rotating-wall vessels. In Vitro Cell Dev Biol. ;28A(1)– pmid doi: /bf View Article PubMed/NCBI Google Scholar


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Morphological differentiation of colon carcinoma cell lines in rotating wall vessels Download PDF EPUB FB2

MORPHOLOGIC DIFFERENTIATION OF COLON CARCINOMA CELL LINES HT AND HTKM IN ROTATING-WALL VESSELS THOMAS J. GOODWIN, J. MILBURN JESSUP, AND DAVID A. WOLF Biomedical Operations and Research Branch, NASA Johnson Space Center (D.

W.); and KRUG Life Sciences, NASA Johnson Space. Get this from a library. Morphological differentiation of colon carcinoma cell lines in rotating wall vessels. [J Milburn Jessup; United States. National Aeronautics and Space Administration.].

Two established human colon adenocarcinoma cell lines, HT, an undifferentiated, and HTKM, a stable, moderately differentiated subline of HT, were grown in new tissue culture bioreactors called Rotating-Wall Vessels (RWVs). RWVs are used in conjunction with multicellular cocultivation to develop a unique in vitro tissue modeling by: Morphologic Differentiation of Colon Carcinoma Cell Lines HT and HTKM in Rotating-Wall Vessels.

The initial phase of this project tested the efficacy of simulated microgravity for the cultivation and differentiation of human colon carcinoma in rotating wall vessels (RWV's) on microcarrier beads.

The RWV's simulate microgravity by randomizing the gravity vector in an aqueous medium under a low shear stress environment in unit : J. Jessup. FINAL REPORT NAG Name of Project: Morphological Differentiation of Colon Carcinoma Cell Lines in Rotating Wall Vessels University Affiliation: Laboratory of Cancer Biology, New England Deaconess Hospital, Harvard Medical School, Boston, MA Principal Investigator: J.

Milburn Jessup, M.D. Address: Pilgrim Road, Boston, MA Title(s): Morphological differentiation of colon carcinoma cell lines in rotating wall vessels.

Final report/ J.M. Jessup. Country of Publication: United States Publisher: Washington, DC: NASA Headquarters, The High Aspect Rotating-Wall Vessel (HARV) described successfully propagated T, a human bladder transitional epithelial cell line, on Cytodex-3 microcarriers in three-dimensional cellular aggregates up to cm in diameter.

Rotating bioreactor studies of cardiac and skeletal tissue constructs Morphologic Differentiation of Colon Carcinoma Cell Lines HT and HTKM in Rotating-Wall Vessels were grown in new.

Biol Cell ; 8. Pinto M, Robine-Leon S, Appay M-D, et al. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture.

Biol Cell ; 9. Leibovitz A, Stinson JC, McCombs WB III, McCoy CE, Masur KC, Mabry ND. The effects of microgravity on differentiation and cell growth in stem cells and cancer stem cells (2D) and three‐dimensional (3D) manner in space and on the rotating wall vessel (RWV), the RPM, CN, or on The subsequent investigation of the organoids revealed that the ratio of stem cells influenced the growth rate of the colon.

Three-dimensional growth and differentiation of ovarian tumor cell line in high aspect rotating wall vessel: morphologic and embryologic considerations.

Cell. The morphogenetic potential of human colon cancer cells was examined by combining cells with foetal rat mesenchyme in organ culture. Out of four cell lines examined, LST cells formed glandular structures composed of a simple columnar or cuboidal epithelium with a lumen in the centre of the cell mass.

Ultrastructurally, these gland-forming LST cells exhibited polarity, with nuclei located. Goodwin TJ, Milburn Jessup and Wolf DA. Morphologic Differentiation of Colon Carcinoma Cell Lines HT and HTKM in Rotating-Wall Vessels. In Vitro Cell Dev Biol, 28 (1), [ PubMed ]. Three-dimensional growth and differentiation of ovarian tumor cell line in high aspect rotating-wall vessel: morphologic and embryologic considerations.

Cell. Biochem. 51, – (). CAS. Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies.

Background: A rotary cell-culture system (RCCS) allows the creation of a microgravity environment of low shear force, high-mass transfer and three-dimensional cell culture of various cell types.

The aim of the study was to evaluate the growth pattern and the secretory function of rabbit lacrimal gland acinar cells in a microgravity environment using an RCCS. T.J. Goodwin et al., "Morphological differentiation of colon carcinoma cell lines HT and HTKM in rotating-wall vessels," In Vitro Cellular and Developmental Biology, 28A, 3.

R.P. Schwarz et al., Rotating bioreactor cell culture apparatus. Goodwin TJ, Jessup JM, Wolf DA. Morphologic differentiation of colon carcinoma cell lines HT and HTKM in rotating-wall vessels. In Vitro Cell Dev Biol Anim ; Baker TL, Goodwin TJ. Three-dimensional culture of bovine chondrocytes in rotating-wall vessels.

In Vitro Cell Dev Biol Anim ; Colon Carcinoma Cell Lines HT and HTKM in Rotating Wall Vessels, Zn Vltro Cell. Dev. Biol. 28A Goodwin, T.J. et a1 (). Reduced Shear Stress: A Major Component in the Ability of Mammalian Tissues to Form Three-Dimensional Assemblies in Stimulated Microgravity.

Cellul. Biochem. Goodwin, T.J. et a1 (). Rotating. Three-Dimensional Growth and Differentiation of Ovarian Tumor Cell Line in High Aspect Rotating Wall Vessel. Becker JL, Prewett TL, Spaulding GF, Goodwin TJ: Morphologic and Embryologic Considerations J Cell BiochemThree Dimensional Modeling of T Human Bladder Carcinoma Cell Line: A New Simulated Microgravity Vessel.

The colon makes up the longest part of the large begins from the caecum at the ileocecal valve and ends in the colon is about meters long and frames the convolute of the small intestine in the abdominal cavity.

However it can be shortened and lie quite flexibly in case of an incomplete rotation of the umbilical loop during embryogenesis.Read "Three‐dimensional growth and differentiation of ovarian tumor cell line in high aspect rotating‐wall vessel: Morphologic and embryologic considerations, Journal of Cellular Biochemistry" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.